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A source of electricity supply in accordance with the rules and standards implemented in the country. Voltages of 110 V and 60 Hz frequencies are generally used in American countries. ELISA an ELISA Analyzer calibration scanner calibration is a specialized process to be performed by a technician or engineer trained, instructed that each manufacturer provides for the effect. To perform the calibration is required to have a set of grey filters, which are mounted on a plate of same geometry to those used for the analysis. Manufacturers supply these filters and can be used to perform calibrations at any wavelength that used the computer.

Calibration filters have at least three values optical density, reestablished within the measuring ranges; one low, one medium and the last, a high value. To perform the calibration is carried out the following process: 1. filter calibration on the computer. 2. Perform a complete calibration filter read. Check if there are differences in readings obtained from channel to channel. If so, invert the filter (180 ) and repeat reading again to rule out that differences may be attributable to the filter itself.

In general, accepted that the instrument does not require calibration, if it is set at two wavelengths. 3. Check if the player is off. If so, proceed with calibration, following routine defined by the manufacturer, especially checking the linearity of the readings keep far more rigorously as possible. 4 If there is filter calibration, check it by placing a color in the wells of a plate solution and then making a complete reading. Then invert the plate 180 and perform a new reading. If both readings are identical average values in each row, the analyser is calibrated. 5. Check if the sliding plate is calibrated, column by column. Place an empty plate and perform readings. Failure to observe mean differences between readings from column to column from the first to the last, you could assume that advancement is calibrated.

Obtaining of information Like part of the project, the U.T.E created by Audiotec and CTA will realise the previous compilation of all the necessary information for the development of the noise maps, as well as the development of the methodologies and techniques necessary for the obtaining of the acoustic data necessary to complete them. In this sense, the Council of Environment considers opportune to begin as rapidly as possible with the data summary to paraadecuar the cartographic information and other types of documentation to the needs of the noise maps and, of this form, to obtain that they are available in 2011 and " to be able to develop the maps in 2012, as it indicates the norm of the Comunidad". In addition, the mentioned Council emphasizes that these works require a high degree of specialization and, therefore, " they only can be carried out by people with a suitable formation and who have the average technicians necesarios". They are requisite to that the organizations are not other people’s that form the U.T.E that has gained east contest on watch, since as much its experience of than 25 years in subjects related more to the noise, like the high qualification of its multidisciplinary human equipment, or the facilities that conform their laboratory of acoustics turn to these organizations into the suitable option for carries out the project. The acoustic information for the creation of the noise maps they will be compiled, like " work of campo" , in the railway streets and routes, as well as gaugings of in motion and railway traffic corresponding to the populations study object. In addition, for the effectiveness of the maps one becomes necessary to contribute data of representative hour intensity, speed average and percentage of vehicles (as much for the light vehicles as for the heavy vehicles), differentiating in any case each one of the periods denominated like:Day (7-19h), Behind schedule (19-23h) and Night (23-7h).